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phosphorylated adenosine monophosphate activated protein kinase pampk thr172  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated adenosine monophosphate activated protein kinase pampk thr172
    Phosphorylated Adenosine Monophosphate Activated Protein Kinase Pampk Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 5418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pampk+rabbit+antibody/Phospho-AMPKalpha+(Thr172)+Rabbit+mAb/10__1016_slash_j__cej__2025__166302-51-8-15
    Average 98 stars, based on 5418 article reviews
    phosphorylated adenosine monophosphate activated protein kinase pampk thr172 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Polyacrylamide Gel Electrophoresis:

    Article Title: Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival.
    Article Snippet: Protein amount was measured using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). .. 50 ug of protein were run on a PAGE gel electrophoresis on 10% Tris-HCl/Glycine SDS-polyacrylamide (456–1034, BioRad, CA), electro-transfer to PVDF membranes (162–0218, BioRad, CA, USA) and hybridized with anti-phospho-ERK1/2 (9106, Cell Signaling Technology, MA, or 4730, Cell Signaling Technology, MA, USA) or anti-total-ERK1/2 antibody (4695, Cell Signaling Technology, MA, USA) 1:1000 in phosphate buffered saline containing 0.1% Tween 20 (PBST) and 5% non-fat dry milk at 4 °C overnight, or hybridized with pAMPK rabbit antibody (2535, Cell Signaling Technology, MA, USA, the antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits) 1:1000 or tAMPK rabbit antibody (2603, Cell Signaling Technology, MA, USA) 1:1000 in tris buffered saline containing 0.1% Tween 20 (TBST) and 5% BSA (A7888, Sigma, MA, USA) at 4 °C overnight, and detected with horseradish-peroxidase conjugated anti-rabbit IgG (7074 S, Cell Signaling Technology, MA) in PBST + 5% non-fat dry milk at room temperature for 60 min using chemiluminescence/autoradiography (Supersignal 34580, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival
    Article Snippet: Protein amount was measured using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). .. 50 ug of protein were run on a PAGE gel electrophoresis on 10% Tris-HCl/Glycine SDS-polyacrylamide (456–1034, BioRad, CA), electro-transfer to PVDF membranes (162–0218, BioRad, CA, USA) and hybridized with anti-phospho-ERK1/2 (9106, Cell Signaling Technology, MA, or 4730, Cell Signaling Technology, MA, USA) or anti-total-ERK1/2 antibody (4695, Cell Signaling Technology, MA, USA) 1:1000 in phosphate buffered saline containing 0.1% Tween 20 (PBST) and 5% non-fat dry milk at 4 °C overnight, or hybridized with pAMPK rabbit antibody (2535, Cell Signaling Technology, MA, USA, the antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits) 1:1000 or tAMPK rabbit antibody (2603, Cell Signaling Technology, MA, USA) 1:1000 in tris buffered saline containing 0.1% Tween 20 (TBST) and 5% BSA (A7888, Sigma, MA, USA) at 4 °C overnight, and detected with horseradish-peroxidase conjugated anti-rabbit IgG (7074 S, Cell Signaling Technology, MA) in PBST + 5% non-fat dry milk at room temperature for 60 min using chemiluminescence/autoradiography (Supersignal 34580, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Nucleic Acid Electrophoresis:

    Article Title: Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival.
    Article Snippet: Protein amount was measured using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). .. 50 ug of protein were run on a PAGE gel electrophoresis on 10% Tris-HCl/Glycine SDS-polyacrylamide (456–1034, BioRad, CA), electro-transfer to PVDF membranes (162–0218, BioRad, CA, USA) and hybridized with anti-phospho-ERK1/2 (9106, Cell Signaling Technology, MA, or 4730, Cell Signaling Technology, MA, USA) or anti-total-ERK1/2 antibody (4695, Cell Signaling Technology, MA, USA) 1:1000 in phosphate buffered saline containing 0.1% Tween 20 (PBST) and 5% non-fat dry milk at 4 °C overnight, or hybridized with pAMPK rabbit antibody (2535, Cell Signaling Technology, MA, USA, the antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits) 1:1000 or tAMPK rabbit antibody (2603, Cell Signaling Technology, MA, USA) 1:1000 in tris buffered saline containing 0.1% Tween 20 (TBST) and 5% BSA (A7888, Sigma, MA, USA) at 4 °C overnight, and detected with horseradish-peroxidase conjugated anti-rabbit IgG (7074 S, Cell Signaling Technology, MA) in PBST + 5% non-fat dry milk at room temperature for 60 min using chemiluminescence/autoradiography (Supersignal 34580, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival
    Article Snippet: Protein amount was measured using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). .. 50 ug of protein were run on a PAGE gel electrophoresis on 10% Tris-HCl/Glycine SDS-polyacrylamide (456–1034, BioRad, CA), electro-transfer to PVDF membranes (162–0218, BioRad, CA, USA) and hybridized with anti-phospho-ERK1/2 (9106, Cell Signaling Technology, MA, or 4730, Cell Signaling Technology, MA, USA) or anti-total-ERK1/2 antibody (4695, Cell Signaling Technology, MA, USA) 1:1000 in phosphate buffered saline containing 0.1% Tween 20 (PBST) and 5% non-fat dry milk at 4 °C overnight, or hybridized with pAMPK rabbit antibody (2535, Cell Signaling Technology, MA, USA, the antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits) 1:1000 or tAMPK rabbit antibody (2603, Cell Signaling Technology, MA, USA) 1:1000 in tris buffered saline containing 0.1% Tween 20 (TBST) and 5% BSA (A7888, Sigma, MA, USA) at 4 °C overnight, and detected with horseradish-peroxidase conjugated anti-rabbit IgG (7074 S, Cell Signaling Technology, MA) in PBST + 5% non-fat dry milk at room temperature for 60 min using chemiluminescence/autoradiography (Supersignal 34580, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Saline:

    Article Title: Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival.
    Article Snippet: Protein amount was measured using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). .. 50 ug of protein were run on a PAGE gel electrophoresis on 10% Tris-HCl/Glycine SDS-polyacrylamide (456–1034, BioRad, CA), electro-transfer to PVDF membranes (162–0218, BioRad, CA, USA) and hybridized with anti-phospho-ERK1/2 (9106, Cell Signaling Technology, MA, or 4730, Cell Signaling Technology, MA, USA) or anti-total-ERK1/2 antibody (4695, Cell Signaling Technology, MA, USA) 1:1000 in phosphate buffered saline containing 0.1% Tween 20 (PBST) and 5% non-fat dry milk at 4 °C overnight, or hybridized with pAMPK rabbit antibody (2535, Cell Signaling Technology, MA, USA, the antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits) 1:1000 or tAMPK rabbit antibody (2603, Cell Signaling Technology, MA, USA) 1:1000 in tris buffered saline containing 0.1% Tween 20 (TBST) and 5% BSA (A7888, Sigma, MA, USA) at 4 °C overnight, and detected with horseradish-peroxidase conjugated anti-rabbit IgG (7074 S, Cell Signaling Technology, MA) in PBST + 5% non-fat dry milk at room temperature for 60 min using chemiluminescence/autoradiography (Supersignal 34580, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival
    Article Snippet: Protein amount was measured using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). .. 50 ug of protein were run on a PAGE gel electrophoresis on 10% Tris-HCl/Glycine SDS-polyacrylamide (456–1034, BioRad, CA), electro-transfer to PVDF membranes (162–0218, BioRad, CA, USA) and hybridized with anti-phospho-ERK1/2 (9106, Cell Signaling Technology, MA, or 4730, Cell Signaling Technology, MA, USA) or anti-total-ERK1/2 antibody (4695, Cell Signaling Technology, MA, USA) 1:1000 in phosphate buffered saline containing 0.1% Tween 20 (PBST) and 5% non-fat dry milk at 4 °C overnight, or hybridized with pAMPK rabbit antibody (2535, Cell Signaling Technology, MA, USA, the antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits) 1:1000 or tAMPK rabbit antibody (2603, Cell Signaling Technology, MA, USA) 1:1000 in tris buffered saline containing 0.1% Tween 20 (TBST) and 5% BSA (A7888, Sigma, MA, USA) at 4 °C overnight, and detected with horseradish-peroxidase conjugated anti-rabbit IgG (7074 S, Cell Signaling Technology, MA) in PBST + 5% non-fat dry milk at room temperature for 60 min using chemiluminescence/autoradiography (Supersignal 34580, Thermo Fisher Scientific, Waltham, MA, USA). ..



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